Molecular genetic techniques for gene manipulation inCandida albicans

Molecular genetic techniques for gene manipulation in Candida albicans

Candida albicans is one of the most common fungal pathogen in humans due to its high frequency as an opportunistic and pathogenic fungus causing superficial as well as invasive infections in immunocompromised patients. An understanding of gene function in C. albicans is necessary to study the molecular basis of its pathogenesis, virulence and drug resistance. Several manipulation techniques hav...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation

Modell Evaluation Using Genetic Manipulation Techniques

Formal Verification is an important area in industry with getting more and more attention. Growing complexity of digital circuits and the use in safety critical systems are the reasons for the need of tools for checking the correctness of designs. In this paper we present a new approach for model evaluation. With our approach we are able to increase the belief of a designer in the right functio...

Rapid molecular techniques for detection of foodborne Bacillus cereus pathogen

Background and Objective: Bacillus cereus is responsible for several outbreaks of foodborne diseases. Therefore, the purpose of the study was to replace routine culture testing, by employing PCR directly from food to detect the bacteria and its enterotoxins. Methods: In the present study, a total of 75 Kibda sandwiches, Sausage sandwiches, Luncheon chicken, Luncheon meat and shawarma sandwiche...

Molecular genetic diagnostic techniques in choroideremia

PURPOSE To optimize and streamline molecular genetics techniques in diagnosing choroideremia (CHM). METHODS PCR primers were designed for exons 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and 15 of the CHM gene. Each PCR protocol was optimized so that all exons could be amplified with the same component ratio and PCR conditions. Sense and antisense primers were tested for their ability to be used as seq...